Research Letter

Successful transcervical embryo transfer in two strains of mice

C. de Milander, M. S. E. Wimmers, J. V. van der Merwe, F. le R. Fourie
Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie | Vol 7, No 1 | a895 | DOI: | © 1988 C. de Milander, M. S. E. Wimmers, J. V. van der Merwe, F. le R. Fourie | This work is licensed under CC Attribution 4.0
Submitted: 17 March 1988 | Published: 17 March 1988

About the author(s)

C. de Milander,, South Africa
M. S. E. Wimmers,, South Africa
J. V. van der Merwe,, South Africa
F. le R. Fourie,, South Africa

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Two strains of mice (a black Balb/C x C5 BL and a white NMRI strain) were stimulated to superovulate and allowed to copulate with males of a similar strain. Blastocysts were obtained from black donor animals after cervical dislocation and flushing of the fallopian tubes 3,5 days after copulation. A total of 460 blastocysts were flushed and 6 blastocysts were transfered to each of the 71 white surrogate individuals which had copulated 2,5 days previously. A total of 51 births were documented with a 31,4% occurrence of black phenotypic animals in the litter.


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