Original Research

Ultrastructural morphometric evaluation of the microtubular system in stimulated B-cells of the pancreas

A. Crous, A. M. de Beer, E. J. Visser
Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie | Vol 11, No 4 | a545 | DOI: https://doi.org/10.4102/satnt.v11i4.545 | © 1992 A. Crous, A. M. de Beer, E. J. Visser | This work is licensed under CC Attribution 4.0
Submitted: 09 July 1992 | Published: 09 July 1992

About the author(s)

A. Crous,, South Africa
A. M. de Beer,, South Africa
E. J. Visser,, South Africa

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Abstract

The intracellular distribution of microtubules in pancreatic B-cells was studied morphometrically to elucidate the positive correlation between microtubular content and the rate of insulin release found by biochemical investigations. Rat islet tissue was glucose stimulated under in vivo and in vitro (isolated islets) conditions and tissue samples taken to represent both phases of the phasic insulin response. Electron micrographs (x40 000) of individual B-cells were assembled into montages to obtain complete cell profiles at high magnification.

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